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曾长军
发布日期:2020年04月20日 点击数:

姓名

曾长军

政治面貌

中共党员

职称/职务

教授/学院教代会主席

导师类型

博导

联系电话

15808153790

电子邮箱

zengchj@sicau.edu.cn

研究方向

1.精液冷冻损伤及抗冻机制

2.濒危野生动物细胞遗传与繁殖生物技术

3.动物遗传资源保存

学术、技术组织任职/头衔


受教育及

工作经历

教育经历:

1.2003-9至2007-9,浙江大学,动物学,博士

2.1998-9至2001-7,四川农业大学,动物遗传育种与繁殖,硕士

3.1994-9至1998-7,四川农业大学,畜牧,学士

工作经历:

1.2015-2至现在,四川农业大学,金沙贵宾厅-优惠大厅,教授

2.2012-1至2013-1,美国加州大学戴维斯分校,再生医学研究所,访问学者

3. 2009-12至2015-1,四川农业大学,金沙贵宾厅-优惠大厅,副教授

4.2007-10至2009-12,四川农业大学,金沙贵宾厅-优惠大厅,讲师

5.2008-4至2012-3,四川农业大学,博士后,导师:李学伟

教学课程

1.主讲本科生《动物繁殖学》、双语课程《Pathways to Pregnancy and Parturition》和《动物繁殖学实验》课程

2.硕士生《动物育种与繁殖技术》和《畜牧生物技术》

人才培养情况

共培养硕士研究生20余人,在读硕士研究生3人,博士研究生3人

科研项目

(近五年)

1.国家自然科学基金面上项目,31872356,miR-26a/PDHX甲基化模式对猪精子代谢和活力的影响,2019-01至2022-12,在研,主持

2.四川省科技厅项目,2017SZ0089-1,朱鹮高质量微卫星标记的筛选,2019-01至2019-12,已结题,主持

3.国家自然科学基金面上项目,31570533,CryomiRs调控大熊猫精子冷冻保存效果的作用机制研究,2016-01至2019-12,63万元,已结题,主持

4.中国大熊猫保护研究中心合作项目,外源激素对大熊猫卵泡发育调控,2018.06-2019.12,主持

代表性论文

(1)Wang WC, Liang K, Chang Y, Ran MX,Zhang Y, Ali MA, Dai DH,Qazi IH,Zhang M,Zhou GB,Yang JD, Angel C, Zeng CJ. miR-26a is Involved in Glycometabolism and Affects Boar Sperm Viability by Targetig PDHX. Cells, 2020, 9(1):146 [IF=5.656]

(2)Ran MX, Zhou YM,Liang K,Wang WC,Zhang Y,Zhang M,Yang JD,Zhou GB,Wu K,Wang CD,Huang Y,Luo B,Qazi IH,Zhang HM,Zeng CJ. Comparative Analysis of MicroRNA and mRNA Profiles of Sperm with Different Freeze Tolerance Capacities in Boar (Sus scrofa) and Giant Panda (Ailuropoda melanoleuca).Biomolecules, 2019, 9: 432

(3)Dai DH, Qazi IH, Ran MX, Liang K, Zhang Y, Zhang M, Zhou GB, Angel C, Zeng CJ. Exploration of miRNA and mRNA Profiles in Fresh and Frozen-Thawed Boar Sperm by Transcriptome and Small RNA Sequencing. Int J Mol Sci, 2019, 20(4):802

(4)Ran MX, Li Y, Zhang Y, Liang K, Ren YN, Zhang M, Zhou GB, Zhou YM, Wu K, Wang CD, Huang Y, Luo B, Qazi IH, Zhang HM, Zeng CJ. Transcriptome Sequencing Reveals the Differentially Expressed lncRNAs and mRNAs Involved in Cryoinjuries in Frozen-Thawed Giant Panda (Ailuropoda melanoleuca) Sperm. Int. J. Mol. Sci. 2018, 19: 3066

(5)Li Y,Li RH,Ran MX,Zhang Y,Liang K,Ren YN,He WC,Zhang M,Zhou GB,Qazi IH,Zeng CJ.High throughput small RNA and transcriptome sequencing reveal capacitation-related microRNAs and mRNA in boar sperm.BMC Genomics,2018,19(1):736

(6)Zhang Y, Dai DH, Chang Y, Li Y, Zhang M, Zhou GB, Peng ZH,Zeng CJ.Cryopreservation of boar sperm induces differential microRNAs expression. Cryobiology, 2017, 76:26-33

(7)Chang Y, Dai DH, Li Y, Zhang Y, Zhang M, Zhou GB, Zeng CJ.Differences in the expression of microRNAs and their predicted gene targets between cauda epididymal and ejaculated boar sperm. Theriogenology, 2016, 86: 2162-2171.

(8)Zeng CJ, Zhang YL, Park SC, Eun JR, Nguyen NT, Tschudy-Seney B, Jung YJ, Theise ND, Zern M, Duan YY. CD34+ Liver Cancer Stem Cells were Formed by Fusion of Hepatobiliary Stem/Progenitor Cells with Hematopoietic Precursor-Derived Myeloid Intermediates.Hepatology, 2015, 62(1, suppl): 548A【会议摘要,SCI收录】

(9)Zeng CJ, Zhang YL, Park SC, Eun JR, Nguyen NT, Tschudy-Seney B, Jung YJ, Theise ND, Zern MA and Duan YY. CD34+ Liver cancer stem cells were formed by fusion of hepatobiliary stem/progenitor cells with hematopoietic precursor-derived myeloid intermediates.Stem Cells and Development, 2015, 24(21): 2467-2478

(10)Park SC,Zeng CJ (#并列第一), Tschudy-Seney B, Nguyen NT, Eun JR, Zhang YL, Ramsamooj R, Zhang YH, Zhao M, Theise ND, Zhou HJ, Zern MA, Duan YY. Clonogenically culturing and expanding CD34+ liver cancer stem cells in vitro.Stem Cells and Development, 2015, 24(13): 1506-1514

(11)Yu FJ,Zeng CJ, Zhang Y, Wang CD, Xiong TY, Fang SG, Zhang HM. Establishment and cryopreservation of a giant panda skeletal muscle-derived cell line.Biopreserv Biobank, 2015, 13(3):195-199

(12)ZhangY,ZengCJ, HeL,DingL, TangKY,PengWP. Selection of endogenous reference microRNA genes for quantitative reverse transcription polymerase chain reaction studies of boar spermatozoa cryopreservation.Theriogenology, 2015, 83(4): 634-641

(13)Zeng CJ, Peng WP, Ding L, He L, Zhang Y, Fang DH, Tang KY. A preliminary study on epigenetic changes during boar spermatozoa cryopreservation.Cryobiology, 2014, 69(1): 119-127

(14)丁丽,方东辉,何莲,彭文培,张燕,曾长军.细胞凋亡相关caspases在猪精子冷冻过程中的表达谱研究.四川农业大学学报, 2014, 32(1): 68-75

(15)彭文培,曾长军.精子发生及冷冻过程中表观遗传调控的研究进展.中国畜牧兽医, 2014, 41 (9): 199-205;

(16)Zeng CJ, Tang KY, He L, Peng WP, Ding L, Fang DH, Zhang Y. Effects of glycerol on apoptotic signaling pathways during boar spermatozoa cryopreservation.Cryobiology, 2014, 68(3): 395-404

(17)Zeng CJ, He L, Peng WP, Ding L, Tang KY, Fang DH, Zhang Y. Selection of optimal reference genes for quantitative RT-PCR studies of boar spermatozoa cryopreservation.Cryobiology, 2014, 68(1):113-21

(18)Park, Su Cheol; Nguyen, Ngoc Tue X.;Zeng, Changjun; et al.Human liver carcinomas originated from CD34+hematopoietic precursors.Hepatology,2012,56(4)(suppl):807A-808A【会议摘要,SCI收录】

(19)丁丽,曾长军.半胱氨酸天冬氨酸特异性蛋白酶介导的细胞凋亡对精子发生发育及冷冻保存的影响.中国畜牧兽医,2013,40(4):138-144

(20)曾长军,张明,赖松家,周光斌,李金月.高等农业院校动物繁殖学教学改革问题与思考.黑龙江动物繁殖,2012,20(3):58-62

(21)Zeng CJ, Ye Q, Fang S G. Establishment and cryopreservation of liver, heart and muscle cell lines derived from the Chinese alligator (Alligator sinensis).Chinese Sci Bull, 2011, 56: 2576-2579

(22)曾长军,叶青,方盛国.扬子鳄肝脏、心脏和肌肉细胞系构建.科学通报,2011,56(22):1780-1783

(23)Wan QH,Zeng CJ, Ni XW, Pan HJ, Fang SG. Giant panda genomic data provide insight into the birth-and-death process of mammalian major histocompatibility complex class II genes.PLoS One,2009,4(1):e4147

(24)Zeng CJ, Pan HJ, Gong SB, Yu JQ, Wan QH, Fang SG. Giant panda BAC library construction and assembly of a 650-kb contig spanning major histocompatibility complex class II region.BMC Genomics,2007,8:315

(25)Zeng CJ, Yu JQ, Pan HJ, Wan QH, Fang SG. Assignment of the giant panda MHC class II gene cluster to chromosome 9q by fluorescence in situ hybridization.Cytogenet Genome Res, 2005,109 (4):533

(26)李媛#,张燕,冉明霞,李荣宏,梁凯,任英男,贺文成,王文灿,曾长军*,猪精子获能前后mRNA和miRNA差异表达研究,中国畜牧兽医学会动物繁殖学分会第十九届学术研讨会论文集:36,中国桂林,2018.08.25-08.27

(27)常玉#,代定卉,李媛,曾长军*,猪附睾尾部精子与新鲜精子间的miRNA差异表达研究,中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会:43,中国南京,2016.08.18-08.21

(28)张燕#,曾长军*,冷冻保存对猪精子miRNA表达水平的影响,中国畜牧兽医学会动物繁殖学分会第十七届学术研讨会文集:106,中国福州, 2014.08.23-08.25

(29)方东辉,彭文培,何莲,丁丽,唐刻意,曾长军*.齐口裂腹鱼(Schizothorax prenanti)两种促性腺激素受体GTHR序列克隆及表达分析,第十七次全国动物遗传育种学术研讨会论文集,558,成都, 2013.10.27-31

(30)彭文培,曹洪涛,方东辉,何莲,丁丽,唐刻意,曾长军*.齐口裂腹鱼(Schizothorax prenanti)促性腺激素GTHβ亚基克隆及组织表达研究.第十七次全国动物遗传育种学术研讨会论文集, 557,成都, 2013.10.27-31

(31)丁丽,彭文培,何莲,方东辉,唐刻意,曾长军*.猪精子冷冻保存过程中Caspase基因表达谱研究,第十七次全国动物遗传育种学术研讨会论文集, 248,成都, 2013.10.27-31

(32)何莲,丁丽,彭文培,方东辉,唐刻意,曾长军*.冷冻保存猪精液实时荧光定量PCR内参基因的筛选.第十七次全国动物遗传育种学术研讨会论文集, 206,成都, 2013.10.27-31

会议报告:

(1)曾长军; Comparative cryoinjury and freeze tolerance of sperm in boarand giant panda by mRNA and miRNA sequencing,第六届全国功能基因组学高峰论坛,四川成都, 2019-10-18至2019-10-20. (会议特邀报告)

(2)曾长军;猪精子冷冻保存过程中的表观遗传变化研究,中国畜牧兽医学会动物繁殖学分会第十七届学术研讨会,中国福州, 2014-8-24至2014-8-25. (会议报告)

各类获奖

1.第九批四川省学术与技术带头人后备人选

2.2010-2011年度四川农业大学“优秀班主任”

3.教育部教育管理信息中心《动物繁殖学》多媒体课件大赛三等奖

专利、新品种、教材、专著等

专利:

一种用于朱鹮的采精方法,2017

专著:

大熊猫进化历史及保护工程,参编,科学出版社,ISBN:9787030549204,2017.11.01

社会服务

与中国大熊猫保护研究中心、成都大熊猫繁育研究基地、峨眉山方好动物繁育有限公司、峨眉山生物资源实验站等保持密切合作,参与大熊猫、朱鹮、犬等动物繁育研究及技术推广工作